Fig. 3

Restriction digestion of BDNF G196A amplicon by NlaIII of representative individuals. The amplicon after digestion in normal condition (i.e. in presence of major allele G) N = major allele (homozygotes) which gives two bands of 216 and 58 bp (Lane 1, 3, 7, 9, 10); A substitution of A in place of G creates new restriction site, so Mut = minor allele (homozygotes) giving three bands of 139, 77 and 58 bp (lane 2) and Heterozygotes having both types of alleles forms 4 bands Het = heterozygotes, which give four bands of 216, 139, 77 and 58 bp (lane 4, 5, 6)