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Fig. 1 | BMC Medical Genetics

Fig. 1

From: KANK1-NTRK3 fusions define a subset of BRAF mutation negative renal metanephric adenomas

Fig. 1

Histologic, immunohistochemical, fluorescence in situ hybridization and molecular genetic analyses findings of metanephric adenoma. a Hematoxylin and eosin (H&E) of translocation negative metanephric adenoma case, 200x total magnification b H&E slide of translocation positive metanephric adenoma case, 200x total magnification c Fluorescence in situ hybridization with probes for chromosomes 9 (green signal) and 15 (orange/red signal) show normal nuclei with two red and two green signals, (original magnifications 100x) d Abnormal fluorescence in situ hybridization utilizing 2 color probes for the t(9;15) showing one green (9p24) and one orange/red (15q24) signal on the normal homologues. The yellow signals represent the fusion of the probes on the abnormal homologues of chromosome 9p24 and 15q24 (original magnifications 100x) e Melting curve analysis of BRAF mutations in metanephric adenoma sample illustrating a sample without a BRAF GTG > GAC (V600E) mutation of codon 600 (nucleotide 1799) f. Melting curve profiles illustrating the detection of a BRAFV600E mutation of the metanephric adenoma sample with a GTG > GAC (V600E) mutation of codon 600 (nucleotide 1799). Green melting curve represents quality control (QC) wild-type (WT), red melting curve represents QC positive for V600E, black curve represents patient samples (E-non-mutated; F-BRAFV600E mutated) are compared, additional melting peaks or changes in peak-area ratios indicate a sequence alteration under the probe (E and F)

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