Table 1 Primer sets used for methylation-specific polymerase chain reaction
From: Effect of DNMT3A polymorphisms on CpG island hypermethylation in gastric mucosa
gene name | methylated forward | methylated reverse | product size |
|---|---|---|---|
p14ARF | 5′-gtgttaaagggcggcgtagc-3’ | 5′-aaaaccctcactcgcgacga-3’ | 122 bp |
p16INK4a | 5′-ttattagagggtggggcggatcgc-3’ | 5′-gaccccgaaccgcgaccgtaa-3’ | 150 bp |
DAPK | 5′-ggatagtcggatcgagttaacgtc-3’ | 5′-ccctcccaaacgccga-3’ | 98 bp |
CDH1 | 5′- ttaggttagagggttatcgcgt-3’ | 5′-taactaaaaattcacctaccgac-3’ | 115 bp |
| Â | unmethylated forward | unmethylated reverse | Â |
p14ARF | 5′-tttttggtgttaaagggtggtgtagt-3’ | 5′-cacaaaaaccctcactcacaacaa-3’ | 132 bp |
p16INK4a | 5′-ttattagagggtggggtggattgt-3’ | 5′-caaccccaaaccacaaccataa-3’ | 151 bp |
DAPK | 5′-ggaggatagttggattgagttaatgtt-3’ | 5′-caaatccctcccaaacaccaa-3’ | 106 bp |
CDH1 | 5′- taattttaggttagagggttattgt-3’ | 5′-cacaaccaatcaacaacaca-3’ | 97 bp |