Fig. 4

Cryosections of the left deltoid muscle biopsy specimen, frozen in isopentane cooled in liquid nitrogen were used for routine histological staining and histochemical techniques. Hematoxylin and Eosin (HE 200x, panel a), Gomori trichrome (GT 200x, panel b) and Periodic Acid Schiff (PAS 200x, panel c) staining revealed unspecific minimal myopathic changes with mild variation in the fiber size and rare atrophic, angulated fibers. On reduced nicotinamide adenine dinucleotide tetrazolium reductase (NADH-TR 200x, panel D and 400x, panel e) unevenness of stain and multiple core-like areas devoid of enzyme activity in both fiber types, but mainly in type I fibers were identified. A nonspecific predominance of type I fibers (dark color) was highlighted by adenosine triphosphatase staining (ATPase pH 4,35,100x, panel f)