Table 1 Predictions of the influence of SNPs in the promoters of the hemoglobin subunit genes on TBP–TATA affinity, as performed by SNP-TATA-Z-tester [31]
Gene | Alleles, WT or mut | DNA sequence, TATA-like box; 5′-3′ | Prognosis, −ln KD |
|---|---|---|---|
HBB | WT rs63750400:G rs281864525:T rs34598529:G rs33980857:A rs33980857:C rs33980857:G rs33981098:C rs33981098:A rs34500389:T rs34500389:A rs33931746:C | cagggctgggcataaaactcagggcagag cagggctgggcataaaaGtcagggcagag cagggctgggcataaaTctcagggcagag cagggctgggcatGaaactcagggcagag cagggctgggcaAaaaactcagggcagag cagggctgggcaCaaaactcagggcagag cagggctgggcaGaaaactcagggcagag cagggctgggcCtaaaactcagggcagag cagggctgggcGtaaaattcagggcagag cagggctgggTataaaactcagggcagag cagggctgggActaaaactcagggcagag cagggctgggcataCaactcagggcagag | 19.20 19.12 19.169 17.849 17.701 18.172 17.673 18.553 18.062 20.175 18.635 18.341 |
HBB_2 | WT rs35518301:G | ggaccagcacaaaaggcagggcagag ggaccagcacGaaaggcagggcagag | 19.29 18.65 |
HBZ | WT rs113180943:T rs1035033590:C | cagctccctgtaataaggggaccctg cagctccctgtaTtaaggggaccctg cagctccctgtaataaggCgaccctg | 20,76 19,28 20,773 |
HBZ_3 | WT rs559282746:G | ggagggtggggcccctatctctcct ggagggtggggcGcctatctctcct | 17.611 17.605 |
HBA1 | WT rs571582665:T | gcgccccaagcataaacctggcgcgc gcgccccaagcataaacTtggcgcgc | 18.36 18.62 |
HBG1 | WT rs573241527:G | agggtgcttccttttattcttcatcc agggtgcttccttttCttcttcatcc | 19.06 17.54 |
HBM | WT rs905730148:A rs937192507:C | ggtgcccggaggctctataaggaggc ggtgcccggagAccctataaggaggc ggtgcccggaggcccCataaggaggc | 19.13 19.23 19.95 |
HBE1 | WT rs768296486:A rs1036491581:G rs150246840:C | aggcaaaaagagagcttgtgtagagc aggcaaaaagagagAttgtgtagagc aggcaaaaagagagaGtgtgtagagc aggcaaaaagagaggttgtgtagCgc | 18.31 18.16 18.01 18.16 |