Fig. 4

Evolutionary conservation of identified G6PD missense variant. a As shown, the second structure and p.Phe373 are located at the end of the beta strand, the green dot depicts the NADP binding site, the orange dot shows the N6-acetyllysine modified residue, and the red dot shows the substrate binding site. bG6PD sequences from different species were aligned using the Clustal Omega in order to investigate the evolutionary conservation of the phenylalanine residue located in the codon. The multiple alignments revealed total conservation of this residue across all species, suggesting that this residue is crucial for the protein functionality