Table 1 Primer sequences of exons of the PDE3A gene
Primer | Sequence |
|---|---|
Exon 1-F | 5′ TTCAGTGAAGAGGGCACCCTAT 3’ |
Exon 1-R | 5′ GTCCGCACGATGGTGGC 3’ |
Exon 2&3-F | 5′ GGAAGCGCTCGTCCAGAT 3’ |
Exon 2&3-R | 5′ AAGGAAATGAGGGACACGGT 3’ |
Exon 4-F | 5′ TGAATCCCGTCACTTCGCTC 3’ |
Exon 4-R | 5′ GGTCTCTCCGTACTGGTGCA 3’ |
Exon 5&6-F | 5′ GGAGCCTGCACCAGTACGG 3’ |
Exon 5&6-R | 5′ GGAGCAGGGCGATGAAAGA 3’ |
Exon 7-F | 5′ TGCCCCAGACCTATCCCCT 3’ |
Exon 7-R | 5′ TTGGTGTCCGAGTGGCTACC 3’ |
Exon 8-F | 5′ TGACTCCACCTGTTATATGTAGCAG 3’ |
Exon 8-R | 5′ AGCCGATGCTTTCCTCAGAG 3’ |
Exon 9&10-F | 5′ CTGAGGAAAGCATCGGCTT 3’ |
Exon 9&10-R | 5′ AAAAGCTTCAAAGAGGCCC 3’ |
Exon 11&12-F | 5′ GAAGACATGGGCCTCTTTGAAG 3’ |
Exon 11&12-R | 5′ ATGTCCATGTGTAAATCCACTGTC 3’ |
Exon 13-F | 5′ TTGGAGTTGATGGCGCTGTAT 3’ |
Exon 13-R | 5′ ACAAGGAAACGGAAATGCTTAA 3’ |
Exon 14-F | 5′ ACTTCGTAGCCAAATTTAATGG 3’ |
Exon 14-R | 5′ GGCCTCTTCATCACCCTGT 3’ |
Exon 15-F | 5′ TGATATCAATGGTCCAGCTAAATG 3’ |
Exon 15-R | 5′ CCCACAATGTGAGAGATGAAGG 3’ |
Exon 16-F | 5′ CACATTGTGGGGCCTCTGT 3’ |
Exon 16-R | 5′ TTCTATGCCTGCCAACCGT 3’ |