Table 1 PCR protocol
From: Identification of ATP2C1 mutations in the patients of Hailey-Hailey disease
94 °C 5 min | |||||
|---|---|---|---|---|---|
I | 94 °C 45 s | 60 °C 45 s | 72 °C 45 s | 2× | 1 |
94 °C 45 s | 59 °C 45 s | 72 °C 45 s | 2× | ||
94 °C 45 s | 58 °C 45 s | 72 °C 45 s | 2× | ||
94 °C 45 s | 57 °C 45 s | 72 °C 45 s | 2× | ||
94 °C 45 s | 56 °C 45 s | 72 °C 45 s | 2× | ||
94 °C 45 s | 55 °C 45 s | 72 °C 45 s | 20× | ||
II | 94 °C 45 s | 55 °C 45 s | 72 °C 45 s | 32× | Other exon |
III | 94 °C 30s | 54 °C 30s | 72 °C 30s | 30× | 5, 7, 12, 27, 28 |