Skip to main content
Fig. 1 | BMC Medical Genetics

Fig. 1

From: A novel ultra-sensitive method for the detection of FGFR3 mutations in urine of bladder cancer patients – Design of the Urodiag® PCR kit for surveillance of patients with non-muscle-invasive bladder cancer (NMIBC)

Fig. 1

a–b Design of Mutation and Methylation PCR assays. The diagram 2a illustrates the Mutation assay with the position of the primers and fluorescent probes used for detection of human FGFR3 mutations (G372C, R248C, S249C, and Y375C) by MASO-PCR. The diagram 2b illustrates the Methylation assay with the position of the primers and fluorescent probes used in quantifying methylation degree of HS3ST2, SEPTIN9, and SLIT2 genes by QM-MSPCR. In both assays, the amplification curves are shown as examples, with Cts values above the established threshold as positive (Case 1) and below the threshold as negative (Case 2)

Back to article page

BMC Medical Genetics

ISSN: 1471-2350

Contact us