Table 2 Primer pairs used for the experimental methods
From: Novel mutations of the SRF gene in Chinese sporadic conotruncal heart defect patients
Primers | Forward (5′ → 3′) | Reverse (5′ → 3′) |
|---|---|---|
SRF-seq | TGCCAGGTAGTGTTTTCTAAGTG | GGCCCCTATTCACCTTCCTT |
Mut-G274D | CACCAACCTGCCGGATACAACCTCCACCA | TGGTGGAGGTTGTATCCGGCAGGTTGGTG |
Mut-G294C | GCAAGTCAGCAGCTGCCCCTCCTTTCC | GGAAAGGAGGGGCAGCTGCTGACTTGC |
RT-SRF | ACTCTCCACCCCGTTCAGAC | TGGTGCACTTGAATGGCCTG |
GAPDH | GAGTCAACGGATTTGGTCGT | TGATTTTGGAGGGATCTCG |