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Table 1 Characteristics of primers used in amplification reactions

From: Hepcidin gene polymorphisms and iron overload in β-thalassemia major patients refractory to iron chelating therapy

Primer

Sequence

5′ → 3′

Position*

Annealing

(°C)

Length

(bp)

Hamp-F

GTCATTTATGGCCAAAAGTTTGCT

4409–4432

61

803

Hamp-R

CTCTCCCATCCCTGCTGC

5194–5211

  1. *Primers positions are assigned according to the NG_011563.2