Case reports: three novel variants in PCCA and PCCB genes in Chinese patients with propionic acidemia

BMC Medical Genetics

Table 3 PCR primers and conditions used for mutation analysis of the PCCB gene

Exon	Sequence(5′-3′)
	Forword	Reverse	Product size(bp)	Annealing temperature(°C)
1	TGGTCAGAGAAGAGCAAGGAC	GGTTATACCCGCCTCCACG	603	60
2	GCCCTTGCTTTGCTTACTAAAT	AGTGTACGGTCGCTCACGC	449	60
3	GCCAAACTCATTAGAAGAAGTATT	TTTTCCCCAAACTACAAGCAAGT	240	56
4	CTGTCTCCAGGGCTCAAGCAA	CCGCAAAGATACTCAATAAGCAC	447	60
5	CTATTAAATATCTGGTCCTTTGTC	ACAATGCGGCAGAGAACAATG	410	58
6	TGTTATCTTATTGTTGTCTTTATCT	ACACCTTATCATCACTATGCTG	332	58
7	GCTGAATCAACTCTAAGGCTGT	CAGTCTTCCCAAATAAGGTCTG	301	60
8	AAGGTATGTATTATGTGGCATTAC	AAGGTATGTATTATGTGGCATTAC	350	60
9	CGTGTCACCCCATTTCCTTTC	CGTGTCACCCCATTTCCTTTC	249	58
10	AGTGTCATTACATCTTATACTTGTC	ATTTAGATTTTTTCCTCTGGTCAT	314	56
11–12	GGATGGCTGCTGAGGACAAAT	CTGCGGGGCTGGGAACAAC	691	60
13	TAGGGCTATTCTTGTTCTTTGTC	GGAGACTTACCAAGGTCTAGGT	358	60
14	CCCACACACAGTGATAATGAGTT	CAAAGGCAAAAAGGCAGTTATGT	276	60
15	CTGAGCAGAAGGTTGAGGGGT	ATTAGGAGATAGATAGGGCATATT	639	60

ISSN: 1471-2350