Case reports: three novel variants in PCCA and PCCB genes in Chinese patients with propionic acidemia

BMC Medical Genetics

Table 2 PCR primers and conditions used for mutation analysis of the PCCA gene

Exon	Sequence(5′-3′)
	Forword	Reverse	Product size(bp)	Annealing temperature(°C)
1	ACTAGCCCTCCAGGTCCTAG	GGAAAGCAAGCGGTGTAGC	612	62
2	TATTGCCTAGAACTACATTTATTGA	ACAGTGTGGAGACGAGAAAGT	276	58
3–4	AACTTGGTGTTTTTTGGTCTTAAA	GTATGTCTCACTTTTTCTGCTTG	362	58
5	TACGACTCTATAAATGATAGGCA	CCTTTGATTTCCAGTAGCGAAT	395	58
6	AAGTGTACTTATTCAAGGGCTC	AATTAAAATTCTATAATCCATCACTA	273	56
7	GTGTTGGCTCAAAAACTGTTGT	TGTGTGTGTGTGTGTATATCCC	380	60
8	ATGAATCGGAGGAGACAGTAG	CTTCCAGAGCAACAAGTAATCA	271	58
9	ATTATTGTTTTCTGCGTTATTGAAC	CATTATGCTTTTGGTATCTGTTTAC	266	58
10	GTCTGACTCTTCTTCTCCTTCTTC	TCTAAAGGCACAACTCACAATCA	293	60
11	AAATAATGTTTTGAGAGGTATGTATA	CAACAGAGTGAGACCCTGTCT	347	60
12	AACTTTAAGAAAATGTTTATGTAATG	ATCTTTATTTAACACTTTATGGAGT	349	60
13	TGTGATTTTTCTTGTTTGTTTCTAT	TACCTCATTGTTTGGCTATACC	295	60
14	TTGATTTTTAAGTACATTCTAAGTTG	TCACTTGTCCTTCAATTTACACC	365	60
15	TTTTCCTATTTTCCAGAAGTTGAA	ACTACAAACTAACATAACGCTGAA	393	60
16	ATGTATTGAAAACTGAACTATCATAA	AATGTGCTGTGCCATATTCTCA	315	60
17	TTTATTGATACCACAAGTTCAGATA	TCAAAATAGAGTGAAATTATGTATTAC	362	60
18	AATAGATGCCCTATAAAATACTTG	ACTTCTCCAAAGACCCATAAGAT	338	60
19	GATGATTCTTAGAGTAGGTGTTTA	GATGAAAAATACATAGAGGTACTAC	367	60
20	AAAATGGCTGCTGCTTTGTATG	CATTAAATGCTCAGGTTAAAACTTA	371	60
21	GTTTTTTGGCTATCGTGAACATTA	CCTAGAATATCATTTGTAAAGGCA	272	60
22	TGATTTAGAATGAATGCTACTTTTGA	CTGTTTAGGGGGCGTCGGT	345	60
23	CACATATTTTGGGGCATTTGACA	GTAGAAGCGAGGGGGAGAGG	304	60

ISSN: 1471-2350