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Fig. 5 | BMC Medical Genetics

Fig. 5

From: Novel deep intronic mutation in the coagulation factor XIII a chain gene leading to unexpected RNA splicing in a patient with factor XIII deficiency

Fig. 5

Effects of the suppression of NMD by CHX and UPF1 siRNA (si-UPF1). A, 293 T cells were transfected with WT and mutant plasmids and were sequentially added into CHX to inhibit translation for 8 h. The relative expression of EGFP-F13A1 was verified via qPCR. B, the expression of UPF1 was analyzed using transfected cells by si-UPF1. C, D, 293 T cells expressing the WT and mutant F13A1 were transfected with UPF1 siRNA for 48 h. EGFP-F13A1 mRNA and protein expression were quantified via qPCR and western blot. **p < 0.05, ***p < 0.01

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