Fig. 4From: Novel deep intronic mutation in the coagulation factor XIII a chain gene leading to unexpected RNA splicing in a patient with factor XIII deficiencyGFP-F13A1 expression in transfected cells. A, 293 T cells were transfected with wt and mut plasmids for 48 h. Fluorescent signals of GFP-F13A1 were detected via fluorescent microscopy. 293 T cells were transfected with wt and mut plasmids. B,C, GFP-F13A1 mRNA and protein expression were quantified via qPCR and western blot***p < 0.01.Back to article page