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Table 1 Primers for PCR and Sanger sequencing used in mutation validation

From: Novel mutations identified in Chinese families with autosomal dominant congenital cataracts by targeted next-generation sequencing

Gene

Transcript

mutation

Forward primer (5′-3′)

Reverse primer (5′-3′)

Product length (bp)

GJA8

NM_005267.4

c.154 T > C

GCAACTTGGAAAGGAGAGGT

ATGTGGCAGATGTAGGTCCT

576

GJA3

NM_021954.3

c.1152_1153insG

GATGACTGAGCAGAACTGGG

CCTGATCTCTCCTCCATCGT

496

BFSP1

NM_001195.3

c.1804G > C

CCAATTGACCAGCAGCCTAT

CTGTCCTCATGAAGCTGACC

623

EPHA2

NM_004431.3

c.1532C > T

AATTCCGAGCCTCAGTTTCC

TGAACTTCCTCACACCACTG

540

HSF4

NM_001538.3

c. 356G > A

CTGCCCCAGTATTTCAAGCA

CCTCCTCTTTGCTCATTCCC

309