- Research article
- Open Access
Increased risk of aspirin-induced gastric mucosal erosion in elderly Chinese men harboring SLCO1B1*1b/*1b while using aspirin and an ACEI or ARB concomitantly
BMC Medical Genetics volume 20, Article number: 183 (2019)
It is well established that long-term use of aspirin can cause gastric mucosal injury. ACEIs and ARBs are inversely related to gastric ulcer development. This study aimed to evaluate the relationship between SLCO1B1 polymorphisms, which can affect ACEI and ARB transport, and gastric mucosal erosion in elderly male Chinese patients with cardiovascular disease who use aspirin.
Patients taking aspirin and an ACEI or ARB concomitantly who had undergone endoscopic screening for gastric erosion were analyzed for SLCO1B1 polymorphisms by a TaqMan assay.
The frequency of the SLCO1B1*1b/*1b diplotype (42% vs. 24%; p = 0.002) was significantly higher in the gastric mucosal erosion group than in the control group. After adjustment for significant factors, SLCO1B1*1b/*1b (OR, 2.64; 95% CI, 1.59–4.17; p < 0.05) was found to be associated with gastric mucosal erosion in aspirin users.
The presence of the SLCO1B1*1b/*1b diplotype may be a risk factor for aspirin-induced gastric mucosal erosion in elderly Chinese men taking aspirin and an ACEI or ARB concomitantly.
Cardiovascular disease is the leading cause of disability and death in elderly Chinese males. Aspirin is a common antiplatelet drug used to prevent and treat ischemic vascular disease. However, antiplatelet therapy results in a higher incidence of gastrointestinal (GI) injury, particularly gastric mucosal ulcers and hemorrhage . GI injury is related to a poor prognosis of cardiovascular disease . Moreover, the mechanisms and genetic influence of antiplatelet-related GI injury remain largely unknown.
It is well established that long-term use of aspirin may result in gastric mucosal injury but that concomitant use of aspirin and an angiotensin-converting enzyme inhibitor (ACEI) or angiotensin II AT-1 receptor blocker (ARB) may attenuate aspirin-induced gastric injury . Their transport from circulating blood to hepatocytes requires the uptake transporter organic anion transporting polypeptide 1B1 (OATP1B1), which is encoded by the solute carrier organic anion transporter 1B1 (SLCO1B1) gene .
Elderly patients are the major demographic using aspirin; thus, identifying the risk factors of early-phase gastric mucosal injury such as gastric mucosal erosion, which can evolve into gastric ulcers but is confined to the gastric mucosa (has not reached the mucosal muscularis), is critical for preventing aspirin side effects. The purpose of this study was to explore the genetic and clinical risk factors associated with gastric mucosal erosion in elderly male Chinese patients with cardiovascular disease taking aspirin and an ACEI or ARB concomitantly.
Materials and methods
This case–control study analyzed 268 elderly male Chinese patients ≥60 years old who took 100 mg of aspirin daily between 2013 and 2018 for ischemic cardiovascular disease and were undergoing annual upper GI endoscopy persistently, including 143 men in the case group and 125 men in the control group according the gastroscope inspection results, at the Second Medical Center of People’s Liberation Army of China (PLA) General Hospital. All participants were also administered ACEIs or ARBs for hypertension. All the endoscopy results of patients were not abnormal prior to 2013, and endoscopy screening findings were stable during antiplatelet therapy. Patients were excluded if they had gastric cancer or other malignant lesions or took other antiplatelet and anticoagulant drugs. This study was approved by the ethics committee of PLA General Hospital. All participants provided written informed consent.
Clinical data collection
Information on the participants was collected from physical examinations and laboratory studies. The physical examination data extracted from the hospital records were age, body mass index, systolic and diastolic blood pressure, current cigarette smoking, alcohol consumption, Helicobacter pylori status, history of gastric ulcer, diabetes mellitus, hyperlipidemia, ischemic cerebrovascular disease, and use of proton-pump inhibitors (PPIs), nonsteroidal anti-inflammatory drugs (NSAIDs), ARBs or ACEIs and statins. H. pylori status was detected using the C13 urea breath test. Diabetes was diagnosed when the subject had a fasting glucose ≥7.0 mmol/L or ≥ 11.1 mmol/L 2 h after oral glucose challenge. Gastric mucosal erosion was defined as erosion injury visible on endoscopy that was limited to the mucosal layer. Hyperlipidemia was defined as low-density lipoprotein cholesterol (LDL-C) ≥3.64 mmol/L or high-density lipoprotein cholesterol (HDL-C) ≤0.91 mmol/L or total triglyceride (TG) ≥1.7 mmol/L or total cholesterol (TC) ≥5.72 mmol/L. Cerebrovascular disease was defined as a previous ischemic stroke based on hospital records. The clinical data were recorded by physicians at the Department of Geriatric Cardiology of PLA General Hospital who were trained by the research team.
Blood samples were collected in prechilled vacutainers after at least 12 h of overnight fasting. The activated partial thromboplastin time (APTT) was determined using an automatic coagulometer (SYSMAX CA-1500, Sysmex Shanghai Ltd., Japan), the platelet count was determined using an automatic hematology analyzer (Nihon Kohden MEK-7222 K, Japan), and TC, LDL-C, glucose, and serum creatinine (SCr) values were determined using an automatic biochemical analyzer (Hitachi 7400, Japan). All testing was performed in the same laboratory by trained personnel following the criteria of the World Health Organization Lipid Reference Laboratories. Renal function was assessed by the estimated glomerular filtration rate (eGFR), which was calculated by the following formula: eGFR (ml/min/1.73 m2) = 175 × standard SCr (mg/dl) – 1.234 × age – 0.179. [standard SCr (mg/dl) = SCr (mg/dl) (detected by an enzymatic method) × 0.795 + 0.29] [5, 6].
DNA isolation and genotyping
Two hundred microliters of venous blood from every patient was extracted and collected into an EDTA anticoagulant tube (Biotend, Shanghai, China). The blood genomic DNA isolation kit (DP318, TIANGEN Biotech, Beijing, China) was used to extract DNA samples, and a Q3000 ultraviolet spectrophotometer (Quawell, San Jose, CA, USA) was employed to detect the concentration of the DNA samples. The genotypes were determined using a TaqMan assay and an ABI Prism Sequence Detector 7000 (Applied Biosystems, Foster City, Calif) according to standard protocols. Genotyping for SLCO1B A388G (rs2306283) (forward primer 5′-TGTTGGTTTTATTGACGGAAGC-3′, reverse primer 5′-CCCACTATCTCAGGTGATGCTCTA-3′) and SLCO1B T521C (rs4149056) (forward primer 5′-TCAACATCGACCTTATCCACTTG-3′, reverse primer 5′-CAATGTAAGAAAGCCCCAATGG-3′) was performed for each sample.
The distribution of continuous variables was tested for normality using the one-sample Kolmogorov-Smirnov test. Data were presented as numbers and frequencies for categorical variables and as means ± standard deviation (SD) for continuous variables. Baseline characteristics and SNP genotypes were compared with the chi-square test for categorical variables and unpaired Student’s t-test for continuous variables. The odds ratio (OR) and 95% confidence interval (CI) were obtained by Mantel–Haenszel statistics and multiple logistic regression analysis to identify the risk or preventive factors after adjustment for the other significant factors determined by univariate analysis. Differences in the genotype frequencies between the two groups and in Hardy–Weinberg equilibrium of allele frequencies at individual loci by comparing the observed and expected genotype frequencies were assessed using the Chi-squared test or Fisher’s exact probability test. All reported p-values were two-tailed, and p < 0.05 was considered statistically significant. Analyses were performed using SPSS software version 19.0 (SPSS IBM Corporation, Armonk, NY, USA).
We enrolled 268 male patients (62–89 years old; average age: 75.2 years). Among these patients, 143 had gastric mucosal erosion, and 125 had normal gastric mucosa. The baseline clinical characteristics according to the endoscopy results are presented in Table 1. Age (78.5 ± 10.1 vs. 71.5 ± 9.6 years, p < 0.001), SCr plasma levels (96 ± 35.3 vs. 88.3 ± 45.8 μmol/ L, p < 0.01), history of gastric ulcer (n = 20, 14% vs. n = 7, 5.6%, p = 0.02) and co-existing diabetes (n = 58, 40.6% vs. n = 30, 24%, p < 0.01) were found to be significantly higher in the gastric erosion group than in the control group. However, eGFR (55.8 ± 23.1 vs. 70.9 ± 18.5 ml/min/1.73 m2, p > 0.001) and PPI use (n = 26, 18.2% vs. n = 63, 50.4%, p < 0.001) were lower in the gastric erosion group than in the control group. These baseline clinical factors contributed to aspirin-induced gastric mucosal erosion to a significantly greater extent in the univariate analysis.
SLCO1B genotype distribution
All subjects were successfully genotyped. The allele frequencies of the polymorphisms did not deviate significantly from those expected under Hardy–Weinberg equilibrium. The frequencies of the T allele (85.7% vs. 72.0%, p < 0.001) and the SLCO1B1 521TT genotype (77.6% vs. 58.4%, p = 0.001) were significantly higher in the gastric erosion group than in the control group. Accordingly, the frequencies of the SLCO1B1 521TC genotype (16.1% vs. 27.2%, p = 0.027) and SLCO1B1 521CC genotype (6.4% vs. 14.4%, p = 0.028) were significantly lower in the gastric erosion group than in the control group (Table 2).
The frequencies of the SLCO1B1*1b haplotype (62.6% vs. 44.8%, p < 0.001) and SLCO1B1*1b/*1b diplotype (42.0% vs. 24.0%, p = 0.002) were significantly higher in the gastric erosion group than in the control group. The frequencies of the SLCO1B1*15 haplotype (11.5% vs. 24.0%, p < 0.001), SLCO1B1*15/*15 diplotype (0.7% vs. 6.4%, p = 0.014) and SLCO1B1*1a/*15 diplotype (3.5% vs. 12.8%, p = 0.005) were significantly lower in the gastric erosion group than in the control group (Tables 3 and 4).
Factors associated with gastric mucosal erosion
After adjusting for significant factors in the univariate analysis, older age (OR 1.33, 95% CI 1.04–2.87, p < 0.05), lower eGFR level (8.04, 3.02–22.6, p < 0.01), history of gastric ulcer (2.41, 1.08–5.01, p < 0.05), decreased use of a PPI (0.18, 0.11–0.31, p < 0.001) and the SLCO1B1*1b/*1b diplotype (2.64, 1.59–4.17, p < 0.05) were significantly associated with gastric mucosal erosion in multiple logistic regression analysis (Table 5).
The curative and side effects of aspirin for the prevention and treatment of ischemic cardiovascular diseases are controversial. The ARRIVE trial found that low-risk populations not only benefit from aspirin for the primary prevention of cardiovascular disease but also experience a slightly increased risk of GI bleeding events . The ASCEND study found that the absolute benefit of aspirin in patients with diabetes was largely counterbalanced by the bleeding hazard . Three other articles published in the New England Journal of Medicine suggested that aspirin did not reduce the risk of cardiovascular events in elderly patients but was associated with a higher bleeding risk than placebo [9,10,11]. To our knowledge, our study is the first to evaluate the relationship between SLCO1B1 polymorphisms affecting the transport of ACEIs or ARBs and aspirin-induced gastric mucosal injury in elderly male Chinese patients. The results showed that the SLCO1B1*1b/*1b diplotype was positively associated with the risk of gastric mucosal erosion in elderly male Chinese patients taking aspirin and an ACEI or ARB concomitantly.
Shiotani et al. considered that the SLCO1B1*1b haplotype and ARBs were both associated with aspirin-induced peptic ulcers in the Japanese population but could not prove the correlation between the SLCO1B1*1b haplotype and ARBs . Additionally, our study emphasized the discovery of early-phase gastric mucosal injury, such as gastric mucosal erosion. The prevention of gastric mucosal erosion is particularly important in the elderly, as it can evolve into a gastric ulcer and even lead to hemorrhage.
Our study also found that the clinical characteristics of increased age, history of gastric ulcers, and no concomitant use of PPIs were risk factors for gastric mucosal erosion. This conclusion is consistent with the finding of another article summarizing the GI bleeding risk factors in aspirin users . Additionally, the level of eGFR decline was found to be associated with gastric mucosal erosion. One possible reason is that aspirin is excreted mainly through the kidneys as a combination of metabolites and free salicylic acid; thus, the degree of gastric mucosal injury may increase as renal function declines. Additionally, the mechanism may be secondary to the local and systemic effects of aspirin .
The renin-angiotensin system functions to regulate vasoconstriction and vasodilatation, in which angiotensin II plays a critical role. When the body receives external stress, angiotensin II, the effects of which can be inhibited by ACEI, is released into the blood stream and GI tract tissue, decreasing the gastric submucosal blood supply via vasoconstriction . The other gastroprotective effects of ACEIs and ARBs might include blocking the sympathetic-adrenergic system-mediated inflammatory cascade of tumor necrosis factor α and intracellular adhesion molecule 1, as well as anti apoptosis and extracellular matrix remodeling by downregulating DDAH/ADMA and EGFR/ERK1/2 signaling .
ACEIs and ARBs are generally transported into human hepatocytes from portal blood to be metabolized, predominantly via OATP1B1 . Among the mutations identified in the OATP1B1 coding gene (SLCO1B), A388G and T521C occur frequently and are common in the Chinese population (allelic frequencies 73 and 14%, respectively) . There are four haplotypes: SLCO1B1*1a (wild type), SLCO1B1*1b, SLCO1B1*5 and SLCO1B1*15, and the SLCO1B1*1b/*1b (GT/GT) and*1a/*1b (AT/GT) diplotypes have been frequently reported in the Chinese population [19, 20]. The T521C single nucleotide polymorphism has been linked to the reduced transport activity of OATP1B1 by affecting the substrate affinity, and the ACEI and ARB concentrations in the blood were reported to be higher in subjects carring the 521C allele . Although how the protein activity of OATP1B1 is affected by the A388G single nucleotide polymorphism is controversial [22, 23], Mwinyi et al. suggested that the activity of OATP1B1 protein transport was significantly higher in *1b/*1b subjects than in *1a/*1a (AT/AT) subjects in vivo, implying that the blood drug concentrations may be lower in *1b/*1b subjects .
Our study found that the SLCO1B1*1b/*1b diplotype is associated with aspirin-induced gastric mucosal erosion in elderly male Chinese patients taking aspirin and an ACEI or ARB concomitantly. This may be because the reduction in ACEI and ARB concentrations in the blood in individuals with the SLCO1B1*1b/*1b diplotype weakens their protective effect against the gastric mucosa.
The major limitation of the present study was that we did not measure the blood concentrations of ACEIs and ARBs. Thus, the relationship between the change in the degree of drug absorption and gastric mucosal erosion was not proven directly. Another limitation was the use of drugs in elderly patients was complicated, making it challenging to assess all factors that might affect gastric mucosal injury, for example, hormone treatments or anticoagulant drugs.
The SLCO1B1*1b/*1b diplotype may be a useful genetic predictor for aspirin-induced gastric mucosal erosion in elderly male Chinese patients taking aspirin and an ACEI or ARB concomitantly. Increased age, a history of gastric ulcers, the extent of eGFR decline, and no concomitant use of PPIs are clinical risk factors for gastric mucosal erosion.
Availability of data and materials
The data that support the findings of this study are available from the corresponding author upon reasonable request.
Angiotensin-converting enzyme inhibitor
Activated partial thromboplastin time
Angiotensin receptor blocker
Estimated glomerular filtration rate
High-density lipoprotein cholesterol
Low-density lipoprotein cholesterol
Nonsteroidal anti-inflammatory drug
Organic anion transporting polypeptide 1B1
People’s liberation army
- SLCO1B1 :
Solute carrier organic anion transporter 1B1
Hernández C, Barrachina MD, Vallecillo-Hernández J, Álvarez Á, Ortiz-Masiá D, Cosín-Roger J, Esplugues JV, Calatayud S. Aspirin-induced gastrointestinal damage is associated with an inhibition of epithelial cell autophagy. J Gastroenterol. 2015;51:691–701.
Tozawa K, Oshima T, Okugawa T, Ogawa T, Ohda Y, Tomita T, Hida N, Fukui H, Hori K, Watari J, Nakamura S, Miwa H. A randomized, double-blind, placebo-controlled study of rebamipide for gastric mucosal injury taking aspirin with or without clopidogrel. Dig Dis Sci. 2014;59:1885–90.
Shiotani A, Nishi R, Yamanaka Y, Murao T, Matsumoto H, Tarumi K, Kamada T, Sakakibara T, Haruma K. Renin-angiotensin system associated with risk of upper GI mucosal injury induced by low dose aspirin. Dig Dis Sci. 2010;56:465–71.
Maeda K, Sugiyama Y. Impact of genetic polymorphisms of transporters on the pharmacokinetic, pharmacodynamic and toxicological properties of anionic drugs. Drug Metab Pharmacokinet. 2008;23:223–35.
Ma YC, Zuo L, Chen JH, Luo Q, Yu XQ, Li Y, Xu JS, Huang SM, Wang LN, Huang W, Xu GB, Wang HY. Modified glomerular filtration rate estimating equation for Chinese patients with chronic kidney disease. J Am Soc Nephrol. 2006;17:2937–44.
Zhang L, Zuo L, Xu G, Wang F, Wang M, Wang S, Lv J, Liu L, Wang H. Community-based screening for chronic kidney disease among populations older than 40 years in Beijing. Nephrol Dial Transplant. 2007;22:1093–9.
Gaziano JM, Brotons C, Coppolecchia R, Cricelli C, Darius H, Gorelick PB, Howard G, Pearson TA, Rothwell PM, Ruilope LM, Tendera M, Tognoni G. ARRIVE Executive Committee. Use of aspirin to reduce risk of initial vascular events in patients at moderate risk ofcardiovascular disease (ARRIVE): a randomised, double-blind, placebo-controlled trial. Lancet. 2018;392:1036–46.
Bowman L, Mafham M, Wallendszus K, Stevens W, Buck G, Barton J, Murphy K, Aung T, Haynes R, Cox J, Murawska A, Young A, Lay M, Chen F, Sammons E, Waters E, Adler A, Bodansky J, Farmer A, McPherson R, Neil A, Simpson D, Peto R, Baigent C, Collins R, Parish S, Armitage J. Effects of aspirin for primary prevention in persons with diabetes mellitus. N Engl J Med. 2018;379:1529–39.
McNeil JJ, Woods RL, Nelson MR, Reid CM, Kirpach B, Wolfe R, Storey E, Shah RC, Lockery JE, Tonkin AM, Newman AB, Williamson JD, Margolis KL, Ernst ME, Abhayaratna WP, Stocks N, Fitzgerald SM, Orchard SG, Trevaks RE, Beilin LJ, Donnan GA, Gibbs P, Johnston CI, Ryan J, Radziszewska B, Grimm R, Murray AM, ASPREE Investigator Group. Effect of aspirin on disability-free survival in the healthy elderly. N Engl J Med. 2018;379:1499–508.
JJ MN, Nelson MR, Woods RL, Tonkin AM, Donnan GA, Nelson MR, Reid CM, Lockery JE, Kirpach B, Storey E, Shah RC, Williamson JD, Margolis KL, Ernst ME, Abhayaratna WP, Stocks N, Fitzgerald SM, Orchard SG, Trevaks RE, Beilin LJ, Johnston CI, Ryan J, Radziszewska B, Jelinek M, Malik M, Eaton CB, Brauer D, Cloud G, Wood EM, Mahady SE, Satterfield S, Grimm R, Murray AM, ASPREE Investigator Group. Effect of aspirin on cardiovascular events and bleeding in the healthy elderly. N Engl J Med. 2018;379:1509–18.
McNeil JJ, Nelson MR, Woods RL, Lockery JE, Wolfe R, Reid CM, Kirpach B, Shah RC, Ives DG, Storey E, Ryan J, Tonkin AM, Newman AB, Williamson JD, Margolis KL, Ernst ME, Abhayaratna WP, Stocks N, Fitzgerald SM, Orchard SG, Trevaks RE, Beilin LJ, Donnan GA, Gibbs P, Johnston CI, Radziszewska B, Grimm R, Murray AM. ASPREE Investigator Group. Effect of aspirin on all-cause mortality in the healthy elderly. N Engl J Med. 2018;379:1519–28.
Shiotani A, Murao T, Sakakibara T, Tarumi K, Manabe N, Kamada T, Kusunoki H, Haruma K. Association of SLCO1B1 1b with peptic ulcer amongst Japanese patients taking low-dose aspirin. Dig Liver Dis. 2012;44:201–5.
Valkhoff VE, Sturkenboom MC, Kuipers EJ. Risk factors for gastrointestinal bleeding associated with low-dose aspirin. Best Pract Res Clin Gastroenterol. 2012;26:125–40.
Yasuda H, Matsuo Y, Sato Y, Ozawa S, Ishigooka S, Yamashita M, Yamamoto H, Itoh F. Treatment and prevention of gastrointestinal bleeding in patients receiving antiplatelet therapy. World J Crit Care Med. 2015;4:40–6.
Heinemann A, Sattler V, Jocic M, Wienen W, Holzer P. Effect of angiotensin II and telmisartan, an angiotensin1 receptor antagonist, on rat gastric mucosal blood flow. Aliment Pharmacol Ther. 1999;13:347–55.
Shahin NN, Abdelkader NF, Safar MM. A novel role of Irbesartan in gastroprotection against indomethacin-induced gastric injury in rats: targeting DDAH/ADMA and EGFR/ERK signaling. Sci Rep. 2018;8:4280.
Yamada A, Maeda K, Kamiyama E, Sugiyama D, Kondo T, Shiroyanagi Y, Nakazawa H, Okano T, Adachi M, Schuetz JD, Adachi Y, Hu Z, Kusuhara H, Sugiyama Y. Multiple human isoforms of drug transporters contribute to the hepatic and renal transport of olmesartan, a selective antagonist of the angiotensin II AT1-receptor. Drug Metab Dispos. 2007;35:2166–76.
Grapci AD, Dimovski AJ, Kapedanovska A, Vavlukis M, Eftimov A, Geshkovska NM, Labachevski N, Jakjovski K, Gorani D, Kedev S, Mladenovska K. Frequencies of single-nucleotide polymorphisms and haplotypes of the SLCO1B1 gene in selected populations of the western balkans. Balkan J Med Genet. 2015;18:5–21.
Oshiro C, Mangravite L, Klein T, Altman R. PharmGKB very important pharmacogene: SLCO1B1. Pharmacogenet Genomics. 2010;20:211–6.
Xu LY, He YJ, Zhang W, Deng S, Li Q, Zhang WX, Liu ZQ, Wang D, Huang YF, Zhou HH, Sun ZQ. Organic anion transporting polypeptide-1B1 haplotypes in Chinese patients. Acta Pharmacol Sin. 2007;28:1693–7.
Gong IY, Kim RB. Impact of genetic variation in OATP transporters to drug disposition and response. Drug Metab Pharmacokinet. 2013;28:4–18.
Prado Y, Saavedra N, Zambrano T, Lagos J, Rosales A, Salazar LA. SLCO1B1 c.388A>G polymorphism is associated with HDL-C levels in response to atorvastatin in Chilean individuals. Int J Mol Sci. 2015;16:20609–19.
Jiang J, Tang Q, Feng J, Dai R, Wang Y, Yang Y, Tang X, Deng C, Zeng H, Zhao Y, Zhang F. Association between SLCO1B1 -521T>C and -388A>G polymorphisms and risk of statin-induced adverse drug reactions: a meta-analysis. Springerplus. 2016;5:1368.
Mwinyi J, Johne A, Bauer S, Roots I, Gerloff T. Evidence for inverse effects of OATP-C (SLC21A6) 5 and 1b haplotypes on pravastatin kinetics. Clin Pharmacol Ther. 2004;75:415–21.
We thank Liwen Bianji, Edanz Editing China (www.liwenbianji.cn/ac) and the professional language editing service AJE (F045-BFC9-2959-096C-1187) for editing the English text of a draft of this manuscript.
This research was supported by grants from the National Key Research Program of China (2017YFC0840100 and 2017YFC0840103) and the Central Health Care Fund (W2015ZD02). The funding bodies played no role in the design of the study and collection, analysis, and interpretation of data and in writing the manuscript.
Ethics approval and consent to participate
The study was approved by the ethics committee of the People’s Liberation Army General Hospital, and each subject provided informed written consent.
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Duan, L., Bai, Y., Li, M. et al. Increased risk of aspirin-induced gastric mucosal erosion in elderly Chinese men harboring SLCO1B1*1b/*1b while using aspirin and an ACEI or ARB concomitantly. BMC Med Genet 20, 183 (2019). https://doi.org/10.1186/s12881-019-0918-4
- Single nucleotide polymorphism
- Gastric mucosa erosion