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Table 1 PCR primers for amplifications of all coding exons of LDL-R

From: Cascade screening for familial hypercholesterolemia-identification of the C308Y mutation in multiple family members and relatives for the first time in mainland China

ExonForwardReverseProduct size
15′-GTGGGAATCAGAGCTTCACG-3’5′-GGATGGAGTGATTATTTGTA-3’410 bp
25′-GATTCTGGCGTTGAGAGACC-3’5′-AGCGGATCACTTGAGACCAG-3’271 bp
35′-GGTCTTTCCTTTGAGTGACAGT-3’5′-CCACTTTGTAATGCCTCCTGG-3’353 bp
45′-TAGAATGGGCTGGTGTTGGG-3’5′-CCAGGGACAGGTGATAGGAC-3’496 bp
55′-GTACAGACACAGGCTGGTCT-3’5′-CCCTCTGGCTTCACAAATCA-3’372 bp
65′-CACACCTGACCTTCCTCCTT-3’5′-TCCCAAAACCCTACAGCACT-3’245 bp
75′-AGTGACCAGTCTGCATCCC-3’5′-GGTTGCCATGTCAGGAAGC-3’211 bp
85′-CCAAGCCTCTTTCTCTCTCTTC-3’5′-GGGATATGAGTCTGTGCAAAGT-3’250 bp
95′-GCACTCTTGGTTCCATCGAC-3’5′-GAGCCCTCATCTCACCTGC-3’285 bp
105′-CAGGTGAGATGAGGGCTCC-3’5′-TCCTTCCTGCTCCCTCCAT-3’333 bp
115′-CAGCTATTCTCTGTCCTCCCA-3’5′-GTGACAGACCAAGACCTCATC-3’306 bp
125′-CAGCACGTGACCTCTCCTTA-3’5′-AGTCTGTGTCTATCCGCCAC-3’229 bp
135′-CCTGTGTCTCATCCCAGTGT-3’5′-CAAGGAGGTTTCAAGGTTGGG-3’244 bp
145′-GCTGATGATCTCGTTCCTGC-3’5′-GACACAGGACGCAGAAACAA-3’232 bp
155′-CACGTGGCACTCAGAAGAC-3’5′-GGACTCCATCTCGTGACCAA-3’296 bp
165′-GGCCTCACTCTTGCTTCTCT-3’5′-CCATCTGACCCCTTAGCTGT-3’299 bp
175′-GTTTTCACTCCAGCCACGG-3’5′-TGGCTTTCTAGAGAGGGTCAC-3’258 bp
185′-TGTTTACCATTTGTTGGCAG-3’5′-TTGGTCTTCTCTGTCTTTG-3’140 bp