From: Autoimmune Polyglandular Syndrome Type 1: a case report
Exon | Primer sequence (5′ → 3′) | Product size (bp) | Annealing temperature (°C) |
---|---|---|---|
1 | F: AAGCGAGGGGCTGCCAGTGTC R: GGGACTATCCCTGGCTCACAG | 258 | 67–60 touch down |
2 | F: TCCACCACAAGCCGAGGAGAT R: AGCTGGGCTGAGCAGGTGACA | 389 | 67–60 touch down |
3 | F: CTGAGGTTGGGACCCTGCTCC R: CTGGAGACCCTGGCTGGCTTC | 231 | 68 |
4 | F: AGAAACCAGAGCCCGGCAAAGG R: AATGACACACCAGGCCAGCACG | 338 | 63 |
5 | F: GCCCAGTGCTGCCTGCTTCTG R: CCATCTTGGAGCCTGGGTCTC | 256 | 64 |
6 | F: TGCAGGCTGTGGGAACTCCAC R: GGGGCATCAAGAGCCAGGCTC | 305 | 65.4 |
7 | F: CATGTGCACCCTCGCTGCTGA R: AGAAAAAGAGCTGTACCCTGTGG | 278 | 65.2 |
8 | F: CACCCCAGCCCAGTCTGCATG R: CTTCAGGGTCAGTGGGTGGAG | 230 | 68 |
9 | F: CTGTCACCCGCTGTCTTGTTC R: GTGGCCATGTGGACAGGAGG | 205 | 63 |
10 | F: CCCAGCAGTCACTGACTCCTG R: CGTAGGTCCTGGGCTCCTTGA | 311 | 68 |
11 | F: CTCGGGTTCGGGTTCAGCTAC R: TGTGGGTGTGGGTTCAGGCCT | 233 | 72–65 touch down |
12 | F: CATACCCCGGAGGTGGCACTC R: CAGCACCGGCATGCATGGAGG | 205 | 68 |
13 | F: AGTGGGACTCCTTGCTGGTTCC R: AGGGACAGCCTGAGTTTCCACG | 487 | 67 |
14 | F: ATGGCCATGATTCTGTGGCTG R: CTCAGCACTCTCTCATCAGAG | 183 | 68 |