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Table 2 Primer sequences and PCR conditions for the amplification of AIRE gene regions

From: Autoimmune Polyglandular Syndrome Type 1: a case report

Exon

Primer sequence (5′ → 3′)

Product size (bp)

Annealing temperature (°C)

1

F: AAGCGAGGGGCTGCCAGTGTC

R: GGGACTATCCCTGGCTCACAG

258

67–60 touch down

2

F: TCCACCACAAGCCGAGGAGAT

R: AGCTGGGCTGAGCAGGTGACA

389

67–60 touch down

3

F: CTGAGGTTGGGACCCTGCTCC

R: CTGGAGACCCTGGCTGGCTTC

231

68

4

F: AGAAACCAGAGCCCGGCAAAGG

R: AATGACACACCAGGCCAGCACG

338

63

5

F: GCCCAGTGCTGCCTGCTTCTG

R: CCATCTTGGAGCCTGGGTCTC

256

64

6

F: TGCAGGCTGTGGGAACTCCAC

R: GGGGCATCAAGAGCCAGGCTC

305

65.4

7

F: CATGTGCACCCTCGCTGCTGA

R: AGAAAAAGAGCTGTACCCTGTGG

278

65.2

8

F: CACCCCAGCCCAGTCTGCATG

R: CTTCAGGGTCAGTGGGTGGAG

230

68

9

F: CTGTCACCCGCTGTCTTGTTC

R: GTGGCCATGTGGACAGGAGG

205

63

10

F: CCCAGCAGTCACTGACTCCTG

R: CGTAGGTCCTGGGCTCCTTGA

311

68

11

F: CTCGGGTTCGGGTTCAGCTAC

R: TGTGGGTGTGGGTTCAGGCCT

233

72–65 touch down

12

F: CATACCCCGGAGGTGGCACTC

R: CAGCACCGGCATGCATGGAGG

205

68

13

F: AGTGGGACTCCTTGCTGGTTCC

R: AGGGACAGCCTGAGTTTCCACG

487

67

14

F: ATGGCCATGATTCTGTGGCTG

R: CTCAGCACTCTCTCATCAGAG

183

68

  1. F forward, R reverse, bp base pair