Table 3 Hybridization components used in molecular hybridization analysis
From: Rapid detection of PAH gene mutations in Chinese people
Hybrid fluid | Ingredient |
|---|---|
Liquid A | 2x SSC, 0.1% SDS |
Liquid B | 0.5 x SSC, 0.1% SDS |
Liquid C | 0.1 mol L Sodium citrate |
Incubating liquid | 0.25 U/mL Streptomycin –POD, 2x SSC, 0.1% SDS |
Color-substrate solution | 0.0015% hydrogen peroxide, 0.1 mg/mL TMB, 0.1 mol/L Sodium citrate |