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Table 2 Primers for PCR amplification and Sanger-sequencing of exons and flanking regions of NPC1 and NPC2 genes

From: Oxysterol/chitotriosidase based selective screening for Niemann-Pick type C in infantile cholestasis syndrome patients

Exon #

Primer

PCR fragment size

Primers Tm

1

NPC1F 5′-GAGCCAGACTCCATAAGTC-3′

NPC1R 5′-AGACCAACTTCCCCAGGAC-3′

466

64

2

NPC2F 5′-GATTGTACTTGAGTGGGCAC-3′

NPC2R 5′-ACAGAGGATCTTGTGATCAG-3′

238

62

3

NPC3F 5′-TGAGGAATGTTGACCTTACTCTAAC-3′

NPC3R 5′-GAAAGCTGAGCATTACCAGTTC-3’

207

64

4

NPC4F 5’-GCTGGCCCTATTATGTGTGAG-3′

NPC4R 5′-ATTTCCTGGCCAATGGAACTG-3’

312

64

5–6

NPC5F 5’-CAGCATTCCAGCATGGTGCATATG-3′

NPC6R 5′-CCATGCAATGGTATTCATGGAGG-3’

1126

64

7–8

NPC7F 5’-GAAGGCAGTAATTAGGGAGG-3′

NPC8R 5′-CCACAAGGTCATCTAGAGTG-3’

1108

62

9

NPC9F 5’-GCTGATTAATCAAGATCTGAGAG-3′

NPC9R 5′-CTCACCTCTGGGTTATGCTC-3’

367

64

10

NPC10F 5’- GCTGAGCTGTATTACTCAACTG-3′

NPC10R 5′-TACCACTTGATGCTAATGAC-3’

292

64

11

NPC11F 5’- CAGAGATACAGTCCATAGCTC-3′

NPC11R 5′- GAGCTGAGATTCAGTCACTG-3’

501

62

12–13

NPC12F 5’- CTTTGTATCGTGAAAGTTAG-3′

NPC13R 5′-CCAGGAGCCATTCACAGTC-3’

931

60

14

NPC14F 5’- CTGCTGTAGAAGGTGGTCTC-3′

NPC14R 5′- GACATGTTCAGGTAGCCAGC-3’

507

64

15–16

NPC15F 5’-CTTGTATCTGTACATGCACATG-3′

NPC16R 5′-GATAATCTGTTTCAGTGAGAGG-3’

472

64

17

NPC17F 5’-GCCCTGTACTCCCTATTAGC-3′

NPC17R 5′-GTTAGAAGCAGGCACTTGCTT-3’

298

62

18–19

NPC18F 5’-GAATCATGAGTCCAGCTGGAG-3′

NPC19R 5′-GGGAGACCCAGCTTTGATATAC-3’

876

62

20

NPC20F 5’- GAAAGTGACATGTGGCTGAAG-3′

NPC20R 5′- GTGGATGCTTATCTGCAATGGC-3’

315

60

21

NPC21F 5’- CAAGACCTGGACTCTCTTGAC-3′

NPC21R 5′- GATATACTGCCCTGTGCTCAG-3’

357

62

22

NPC22F 5’- AGGAGTCTGACCACTTGGCAGT-3′

NPC22R 5′- ACATGGAATCTAAGACAGCC-3’

382

64

23

NPC23F 5’-GAGGCCTTGTAAGTCCAATGGG-3′

NPC23R 5′-GTACAGGATCCAGACTCTTCAG-3’

312

64

24

NPC24F 5’- GAGAAATCCTTGTAAGGAAG-3′

NPC24R 5′- GATGAGAACTCTTACCTATG-3’

228

64

25

NPC25F 5’-TTCCAAAGTGGGATTACAGGCGTG-3′

NPC25R 5′-GACCGACCCTTAGACACAGTTCAG-3’

183

64

1

NC2-1F 5’-AGACTGCAGGCTTCTGGGCCTGAG-3′

NC2-1R 5′-CCAGCCCCAGGGGTCTCAGCGC-3’

332

64

2

NC2-2F 5’-AGCAGAGCACCTTCCCATTAG G-3′

NC2-2R 5′-CTCCCCTCCATTCCCATGCTT A-3’

256

64

3

NC2-3F 5’-ATGCTGTTGCTTGGGATTATTTC-3′

NC2-3R 5′-CCCATCTCTGCTTCTTGCCCACT-3’

339

62

4

NC2-4F 5’-GGCTGTAAGCTGTGCCCACATGCT-3′

NC2-4R 5′-CTGGACCTTCCTTACTCCGACAG-3’

552

62

5

NC2-5F 5’-TAACTTGCCCTAGGGTTATTGC- 3′

NC2-5R 5′-GTGCACTCTGGGACCACGGAACT-3’

505

62