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Table 2 Primers for PCR amplification and Sanger-sequencing of exons and flanking regions of NPC1 and NPC2 genes

From: Oxysterol/chitotriosidase based selective screening for Niemann-Pick type C in infantile cholestasis syndrome patients

Exon # Primer PCR fragment size Primers Tm
1 NPC1F 5′-GAGCCAGACTCCATAAGTC-3′ NPC1R 5′-AGACCAACTTCCCCAGGAC-3′ 466 64
2 NPC2F 5′-GATTGTACTTGAGTGGGCAC-3′ NPC2R 5′-ACAGAGGATCTTGTGATCAG-3′ 238 62
3 NPC3F 5′-TGAGGAATGTTGACCTTACTCTAAC-3′ NPC3R 5′-GAAAGCTGAGCATTACCAGTTC-3’ 207 64
4 NPC4F 5’-GCTGGCCCTATTATGTGTGAG-3′ NPC4R 5′-ATTTCCTGGCCAATGGAACTG-3’ 312 64
5–6 NPC5F 5’-CAGCATTCCAGCATGGTGCATATG-3′ NPC6R 5′-CCATGCAATGGTATTCATGGAGG-3’ 1126 64
7–8 NPC7F 5’-GAAGGCAGTAATTAGGGAGG-3′ NPC8R 5′-CCACAAGGTCATCTAGAGTG-3’ 1108 62
9 NPC9F 5’-GCTGATTAATCAAGATCTGAGAG-3′ NPC9R 5′-CTCACCTCTGGGTTATGCTC-3’ 367 64
10 NPC10F 5’- GCTGAGCTGTATTACTCAACTG-3′ NPC10R 5′-TACCACTTGATGCTAATGAC-3’ 292 64
11 NPC11F 5’- CAGAGATACAGTCCATAGCTC-3′ NPC11R 5′- GAGCTGAGATTCAGTCACTG-3’ 501 62
12–13 NPC12F 5’- CTTTGTATCGTGAAAGTTAG-3′ NPC13R 5′-CCAGGAGCCATTCACAGTC-3’ 931 60
14 NPC14F 5’- CTGCTGTAGAAGGTGGTCTC-3′ NPC14R 5′- GACATGTTCAGGTAGCCAGC-3’ 507 64
15–16 NPC15F 5’-CTTGTATCTGTACATGCACATG-3′ NPC16R 5′-GATAATCTGTTTCAGTGAGAGG-3’ 472 64
17 NPC17F 5’-GCCCTGTACTCCCTATTAGC-3′ NPC17R 5′-GTTAGAAGCAGGCACTTGCTT-3’ 298 62
18–19 NPC18F 5’-GAATCATGAGTCCAGCTGGAG-3′ NPC19R 5′-GGGAGACCCAGCTTTGATATAC-3’ 876 62
20 NPC20F 5’- GAAAGTGACATGTGGCTGAAG-3′ NPC20R 5′- GTGGATGCTTATCTGCAATGGC-3’ 315 60
21 NPC21F 5’- CAAGACCTGGACTCTCTTGAC-3′ NPC21R 5′- GATATACTGCCCTGTGCTCAG-3’ 357 62
22 NPC22F 5’- AGGAGTCTGACCACTTGGCAGT-3′ NPC22R 5′- ACATGGAATCTAAGACAGCC-3’ 382 64
23 NPC23F 5’-GAGGCCTTGTAAGTCCAATGGG-3′ NPC23R 5′-GTACAGGATCCAGACTCTTCAG-3’ 312 64
24 NPC24F 5’- GAGAAATCCTTGTAAGGAAG-3′ NPC24R 5′- GATGAGAACTCTTACCTATG-3’ 228 64
25 NPC25F 5’-TTCCAAAGTGGGATTACAGGCGTG-3′ NPC25R 5′-GACCGACCCTTAGACACAGTTCAG-3’ 183 64
1 NC2-1F 5’-AGACTGCAGGCTTCTGGGCCTGAG-3′ NC2-1R 5′-CCAGCCCCAGGGGTCTCAGCGC-3’ 332 64
2 NC2-2F 5’-AGCAGAGCACCTTCCCATTAG G-3′ NC2-2R 5′-CTCCCCTCCATTCCCATGCTT A-3’ 256 64
3 NC2-3F 5’-ATGCTGTTGCTTGGGATTATTTC-3′ NC2-3R 5′-CCCATCTCTGCTTCTTGCCCACT-3’ 339 62
4 NC2-4F 5’-GGCTGTAAGCTGTGCCCACATGCT-3′ NC2-4R 5′-CTGGACCTTCCTTACTCCGACAG-3’ 552 62
5 NC2-5F 5’-TAACTTGCCCTAGGGTTATTGC- 3′ NC2-5R 5′-GTGCACTCTGGGACCACGGAACT-3’ 505 62