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Table 2 Primers for PCR amplification and Sanger-sequencing of exons and flanking regions of NPC1 and NPC2 genes

From: Oxysterol/chitotriosidase based selective screening for Niemann-Pick type C in infantile cholestasis syndrome patients

Exon # Primer PCR fragment size Primers Tm
1 NPC1F 5′-GAGCCAGACTCCATAAGTC-3′
NPC1R 5′-AGACCAACTTCCCCAGGAC-3′
466 64
2 NPC2F 5′-GATTGTACTTGAGTGGGCAC-3′
NPC2R 5′-ACAGAGGATCTTGTGATCAG-3′
238 62
3 NPC3F 5′-TGAGGAATGTTGACCTTACTCTAAC-3′
NPC3R 5′-GAAAGCTGAGCATTACCAGTTC-3’
207 64
4 NPC4F 5’-GCTGGCCCTATTATGTGTGAG-3′
NPC4R 5′-ATTTCCTGGCCAATGGAACTG-3’
312 64
5–6 NPC5F 5’-CAGCATTCCAGCATGGTGCATATG-3′
NPC6R 5′-CCATGCAATGGTATTCATGGAGG-3’
1126 64
7–8 NPC7F 5’-GAAGGCAGTAATTAGGGAGG-3′
NPC8R 5′-CCACAAGGTCATCTAGAGTG-3’
1108 62
9 NPC9F 5’-GCTGATTAATCAAGATCTGAGAG-3′
NPC9R 5′-CTCACCTCTGGGTTATGCTC-3’
367 64
10 NPC10F 5’- GCTGAGCTGTATTACTCAACTG-3′
NPC10R 5′-TACCACTTGATGCTAATGAC-3’
292 64
11 NPC11F 5’- CAGAGATACAGTCCATAGCTC-3′
NPC11R 5′- GAGCTGAGATTCAGTCACTG-3’
501 62
12–13 NPC12F 5’- CTTTGTATCGTGAAAGTTAG-3′
NPC13R 5′-CCAGGAGCCATTCACAGTC-3’
931 60
14 NPC14F 5’- CTGCTGTAGAAGGTGGTCTC-3′
NPC14R 5′- GACATGTTCAGGTAGCCAGC-3’
507 64
15–16 NPC15F 5’-CTTGTATCTGTACATGCACATG-3′
NPC16R 5′-GATAATCTGTTTCAGTGAGAGG-3’
472 64
17 NPC17F 5’-GCCCTGTACTCCCTATTAGC-3′
NPC17R 5′-GTTAGAAGCAGGCACTTGCTT-3’
298 62
18–19 NPC18F 5’-GAATCATGAGTCCAGCTGGAG-3′
NPC19R 5′-GGGAGACCCAGCTTTGATATAC-3’
876 62
20 NPC20F 5’- GAAAGTGACATGTGGCTGAAG-3′
NPC20R 5′- GTGGATGCTTATCTGCAATGGC-3’
315 60
21 NPC21F 5’- CAAGACCTGGACTCTCTTGAC-3′
NPC21R 5′- GATATACTGCCCTGTGCTCAG-3’
357 62
22 NPC22F 5’- AGGAGTCTGACCACTTGGCAGT-3′
NPC22R 5′- ACATGGAATCTAAGACAGCC-3’
382 64
23 NPC23F 5’-GAGGCCTTGTAAGTCCAATGGG-3′
NPC23R 5′-GTACAGGATCCAGACTCTTCAG-3’
312 64
24 NPC24F 5’- GAGAAATCCTTGTAAGGAAG-3′
NPC24R 5′- GATGAGAACTCTTACCTATG-3’
228 64
25 NPC25F 5’-TTCCAAAGTGGGATTACAGGCGTG-3′
NPC25R 5′-GACCGACCCTTAGACACAGTTCAG-3’
183 64
1 NC2-1F 5’-AGACTGCAGGCTTCTGGGCCTGAG-3′
NC2-1R 5′-CCAGCCCCAGGGGTCTCAGCGC-3’
332 64
2 NC2-2F 5’-AGCAGAGCACCTTCCCATTAG G-3′
NC2-2R 5′-CTCCCCTCCATTCCCATGCTT A-3’
256 64
3 NC2-3F 5’-ATGCTGTTGCTTGGGATTATTTC-3′
NC2-3R 5′-CCCATCTCTGCTTCTTGCCCACT-3’
339 62
4 NC2-4F 5’-GGCTGTAAGCTGTGCCCACATGCT-3′
NC2-4R 5′-CTGGACCTTCCTTACTCCGACAG-3’
552 62
5 NC2-5F 5’-TAACTTGCCCTAGGGTTATTGC- 3′
NC2-5R 5′-GTGCACTCTGGGACCACGGAACT-3’
505 62