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Fig. 3 | BMC Medical Genetics

Fig. 3

From: Overexpression of MicroRNA-148b-3p stimulates osteogenesis of human bone marrow-derived mesenchymal stem cells: the role of MicroRNA-148b-3p in osteogenesis

Fig. 3

Increased expression of miR-148b-3p in human BM-MSCs was potentially mediated by LV enhanced osteogenic differentiation. a After 21 days mineral nodules depositions were detected with ARS staining. b The stained cells were eluted using 10% acetic acid and the absorbance of extracted stain was measured at 405 nm to quantify the mineralization level. c ALP production was evaluated with BCIP/NBT kit as dark purples deposits. d After 14 days ALP activity was quantified with pNPP substrate system.****P < 0.0001

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