Fig. 3

Overexpression of AP-1 transcription factors or stimulation with PMA/Ionomycin increases expression of the FGF7 gene in fibroblast cells. a and b Messenger RNA expression of the FGF7 gene in fibroblast cell lines carrying various genotypes at the variant rs12905203 at resting- and stimulated-conditions. c mRNA expression of the FGF7 gene in fibroblast cell lines at resting and stimulated conditions. d Protein expression of the KGF in fibroblast cell lines carrying homozygous risk and non-risk variants was determined using western blotting with an anti-KGF antibody. e and f The protein expression of KGF in fibroblast cell lines were normalized to the beta-actin controls for resting- (e) and stimulated (f) conditions. Three independent experiments were performed, and the differences in protein expression of the KGF were calculated by paired Student’s t-test. g Fibroblast cells were transfected with c-Fos, c-Jun, or c-Fos/c-Jun expression vectors. The expression of the KGF protein was determined by western blotting with an antibody against KGF. h We performed densitometric quantification of c-Fos, c-Jun, and KGF in three independent experiments. The statistical differences between groups were calculated by using Student’s t-test