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Table 1 Primers used for GRIN1 gene sequencing

From: Association between polymorphisms in the GRIN1 gene 5′ regulatory region and schizophrenia in a northern Han Chinese population and haplotype effects on protein expression in vitro

Primer name

Annealing temperature (°C)

Primer sequence (5′ → 3′)

F (−2334 — -2313)

60

5’ AGCTTGGGGACGCACATACGGT 3’

R1 (+ 64 — + 86)

60

5’ AATGTTGACGATCTTGGGGTCGC 3’

R2 (− 700 — -676)

5’ GATCACCTGCCCGTACCCTGCTGCA 3’

R3 (− 1515 — -1491)

5’ GTCGTCACCCACAGTCAGCGATATT 3’

  1. F indicates the forward primer and R indicates the reverse primer. F and R1 are PCR amplification primers, and F, R1, R2, and R3 are Sanger sequencing primers. The position of the primer is in parentheses (with ATG + 1).