Fig. 3

a: Graphical representation of amino acid multiple sequence alignment among different species. It shows degree of conservation for amino acids 447–465 of SEPN1 protein. Position 460 (serine) indicates the mutated residue in our study. It has middle conservation degree (2 bits) and also shows that it is replaced by two other amino acids, i.e., glycine and proline in other species but not by phenylalanine. A logo represents the height of each letter proportional to the observed frequency of the corresponding amino acid. The overall height of each stack is proportional to the sequence conservation, measured in bits, at that position. The maximum sequence conservation per site is log₂ 20 ≈ 4.32 bits for proteins. The protein sequence alignment was performed for the following species: Homo sapiens (Q9NZV5), Gorilla gorilla gorilla (G3R759), Pongo abelii (H2N8I1), Papio anubis (A0A2I3MR13), Rhinopithecus roxellan (A0A2K6RVS9), Sus scrofa (A1E950), Bos taurus (F1MD36), Myotis lucifugus (G1P3X6), Dipodomys ordii (A0A1S3GIF9), Equus caballus (F7BM99), Loxodonta africana (G5E785), and Mus musculus (D3Z2R5). b: Multiple protein sequence alignment of SEPN1 across different kingdoms