Table 1 List of APOBEC3 primers designed; primer name, sequence and product size are indicated
Name | Sequence (5′-3′) | Product size |
|---|---|---|
A3D (12.1 kb) | ||
A3D Forward primer | AGGAAGCCTCGCTCTCTCA | 12,069 bp |
A3D Forward primer | CAGGCAGGGTCTTGATCTGT | |
A3D Amplicon 1F | AAAAAGAGGGAGACTGGGACAAGCGTATCTAAGA | 4,300 bp |
A3D Amplicon 1R | GAGTGTGGGTGAGGGGGTGTAACCATGAG | |
A3D Amplicon 2F | AGCTAGGAGAGGTCACCCTG | 3,188 bp |
A3D Amplicon 2F | CAGGAGGCTAGAAGAGACAGACCATGAGGC | |
A3F (13.31 kb) | ||
A3F 1st round f | ACCAGAAAGAGGGTGAGAGACTGAGGAAGATAAAG | 13,142 bp |
A3F 1st round rv | AGCCATTTATTGCAGAAGCTATGGATAAAGCTGGT | |
A3F Amplicon 1 f | ACCAGAAAGAGGGTGAGAGACTGAGGAAGATAAAG | 4,918 bp |
A3F Amplicon 1 rv | GGGTGAGGGGTGTAACCATG | |
A3F Amplicon 2 f | TTCAGAAACCCGATGGAGGC | 4,478 bp |
A3F Amplicon 2 rv | AGCCATTTATTGCAGAAGCTATGGATAAAGCTGGT | |
A3G (10.74 kb) | ||
A3G 1st round f | TGTTAACCAGAGGCTGCTCTTCCCAGG | 11,852 bp |
A3G 1st round rv | TCCCTGGGACTCAGCTCC | |
A3G Amplicon 1 f | ATTTGTCCCCAGCTCTGTGG | 3,231 bp |
A3G Amplicon 1 rv | AGAGGACCTGGTCTGGAACA | |
A3G Amplicon 2 f | CAAGGGAGGAAGCGTGGAG | 3,908 bp |
A3G Amplicon 2 rv | TGCATTGCTTTGCTGGTGTC | |
A3H (6.8 kb) | ||
APOBEC3 H forward primer full length | TCTGTTGCACAGAAACACGATGG | 3522bp |
APOBEC3 H reverse primer full length | CAACTGACATGCCCCAGGG | |
APOBEC3 H forward primer Exon2 (A3HfE2) | TCTGTTGCACAGAAACACGATGG | 452bp |
APOBEC3 H Reverse primer Exon 2(A3HrE2) | TTCCCGAAGTAGTGACTGAGC | |
APOBEC3 H forward primer Exon 3 &4(A3HfE3/4 | GCCACGCACTAGAAAGTTCAC | 934bp |
APOBEC3 H Reverse primer Exon 3&4(A3HrE3/4) | ACAGTGCCTCACCTTTATCC | |