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Fig. 2 | BMC Medical Genetics

Fig. 2

From: De novo mutations in SCN1A are associated with classic Rett syndrome: a case report

Fig. 2

The relative expression level of MECP2 (the alternative transcripts coding for isoforms MeCP2_E1) in cultured fibroblasts of three female controls and Case 1. The MECP2 gene expression analysis was performed on mRNA isolated from her fibroblasts, using Applied Biosystems 7500 Fast Real-Time PCR System (ABI, Foster City, CA), with pre-designed assay (TaqMan MGB probe HS01598237). GAPDH served as an endogenous control. The RQmin and RQmax with the confidence interval set at 95%:Female1; RQ Min 0.77, RQ Max 1.29, Female2; RQ Min 0.85, RQ Max 1.14, Female3; RQ Min 0.787, RQ Max 0,99, Patient; RQ Min 0.07, RQ Max 0,13

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