Fig. 5

Protein expression levels of WT-CRYAA and R12L-CRYAA transfected into HEK293T cells. Western blots were performed with the anti-αA crystallin antibody. β-actin was used as the loading control. (a) The mutant R12L-CRYAA in HEK293T cells had a greatly increased level of protein expression when compared with the wild-type allele. Moreover, a large amount of the mutant protein aggregated in the precipitate where the wild-type protein was not detected. Expression levels of the β-actin loading control in both wild-type and the mutant proteins were comparable. (b) Each bar represents the mean ± standard deviation of three individual experiments. The difference was significance (p < 0.01)