Fig. 2
From: Potential role for nectin-4 in the pathogenesis of pre-eclampsia: a molecular genetic study
Semi-quantitative western blot analysis of nectin-4. a The same blot was incubated against a nectin-4 antibody (upper panel) and a β-actin antibody (lower panel). Rabbit polyclonal anti-human nectin-4 antibodies we raised in this study were used. Lanes 1–4, lysates of placental tissue from uncomplicated normotensive pregnancies; lanes 5–7, lysates of placental tissue from pre-eclamptic pregnancies; lane R, recombinant nectin-4. The data of western blot using commercial goat polyclonal antibodies was shown in Additional file 1: Figure S1. b Semi-quantitative analysis of the blot described in A. Data obtained from uncomplicated normotensive pregnancy (open bars) versus severe pre-eclampsia (grey bars) were compared. The left and right panels indicate the data for the upper bands (open triangle), and lower bands (close triangle), respectively. Each bar represents the mean value. Error bars indicate the standard deviation