Table 1 Primer sequences for PCR, fragment analysis and Sanger sequencing

c.35delG

(GJB2)

(13:20189547)

F: 5′-GGTGAGGTTGTGTAAGAGTTGG-3′

R: 5’-CTGGTGGAGTGTTTGTTCC*CAC-3’

PCR-mediated site-directed mutagenesis (PSDM) with use of Bsc4 I

D13S141^b

(13:20150320–20,150,445)

F: 5’-GTCCTCCCGGCCTAGTCTTA-3’ (6-FAM)

R: 5’-ACCACGGAGCAAAGAACAGA-3’

Fragment analysis (GeneScan 500 LIZ) on ABI 3130XL (Applied Biosystems)

D13S175^b

(13:20274367–20,274,479)

F: 5’-TATTGGATACTTGAATCTGCTG-3’ (PET)

R: 5’-TGCATCACCTCACATAGGTTA-3’

D13S1853^b

(13:20466607–20,466,800)

F: 5’- CAGACTGGCACAAACTTAACTG −3’ (6-FAM)

R: 5’- TGTACATCTCTTCTTACATTCATGT − 3’

rs3751385

(13:20188817)

F: 5’-GGCTGGTGAAGTGCAACG-3′

R: 5’-GTAAGCAAACAAACTTTTGAAGTAG-3’

PCR-RFLP analysis with use of Nhe I