Fig. 4
Comparison of GsĪ±-protein levels in OK cells transfected with WT or mutant GNAS constructs by western blot analysis. Cells transfected with the empty plasmid vector (pcDNA3.1) and non-transfected control (NC) were used as controls. The bands were blotted with anti-GNAS antibody. GAPDH was used as an internal control. The protein expression of the mutant GsĪ±-239D was similar to that the WT GNAS