Fig. 3

Subcellular distribution of WT and p.H311Y mutant FOXL2. Nuclear (Nuc) and cytosolic (Cyto) extracts, as well as whole cell lysate (WCL) of cells transfected with WT or p.H311Y FOXL2 were subjected to Western blotting. Both WT and mutant FOXL2 showed nuclear localization. Lamin B1 and tubulin were used as markers of nuclear and cytoplasmic fractions, respectively