Table 1 Primers sequences and the expected product size
Gene of interest | Forward & reverse primers | Expected product size | Annealing T |
|---|---|---|---|
ERCC1 C118T | F:5’GCAGAGCTCACCTGAGGAAC3’ | 208 bp (Won et al., 2010) | 65 °C 1 min |
R:5’GAGGTGCAAGAAGAGGTGGA3’ | |||
ERCC1 C8092A | F:5’CAGAGACAGTGCCCCAAGAG3’ | 161 bp (Zhou et al., 2004) | 63 °C 45 s |
R:5’GGGCACCTTCAGCTTTCTTT3’ | |||
ERCC2 G312A | F:5’CAGCTCATCTCTCCGCAGGATCAA3’ | 165 bp (Mechanic et al., 2005) | 61 °C 45 s |
R:5’GTCGGGGCTCACCCTGCAGCACTTCCT3’ | |||
ERCC2 A751C | F:5’TCAAACATCCTGTCCCTACT3’ | 344 bp (Sturgis et al., 2000) | 60 °C 35 s |
R:5’CTGCGATTAAAGGCTGTGGA3’ |