Fig. 3
From: PHACTR1 splicing isoforms and eQTLs in atherosclerosis-relevant human cells
Presence of PHACTR1 protein in vascular smooth muscle cells. We tested for the presence of the PHACTR1 protein by immunoblotting using a custom anti-PHACTR1 antibody. The first and second lane, using teloHAEC protein extracts treated or not with a short interfering RNA (siRNA) against PHACTR1, serve as control to demonstrate the specificity of this anti-PHACTR1 antibody. Although we did not detect PHACTR1 in human aortic smooth muscle cells (HASMC), there is weak but specific band corresponding to PHACTR1 in human coronary artery smooth muscle cells (HCASMC). The expected 64 kDa PHACTR1 protein migrated with an apparent molecular weight of 74 kDa. GAPDH was used as loading control