Table 2 Restriction endonucleases that were used with their palindromic recognition sequences and resulting restriction patterns
ACADS variant | Restriction endonuclease (recognition sequence) | Restriction pattern |
|---|---|---|
c.310_312delGAG | BseRI (GAGGAG(N)10^) | 51 bp + 168 bp + 177 bp (wt)/ 177 bp + 219 bp (mut) |
c.511C>T | HpaII (C^CGG) | 98 bp + 141 bp + 80 bp (wt)/ 239 bp + 80 bp (mut) |
c.625G>A | Hpy188I (TCN^GA) | 71 bp + 294 bp + 10 bp (wt)/ 71 bp + 26 bp + 268 bp + 10 bp (mut) |
c.1138C>T | BsrBI (CCG^CTC) | 115 bp + 179 bp (wt)/ 294 bp (mut) |