Fig. 2
Protein purification and in vitro N-terminal acetyltransferase assays (a). Size exclusion chromatography of His/MBP-NAA10-V111G and His/MBP-NAA10-WT. Both the void volume (peak 1), and the monomeric peak at approximately 74 ml (peak 2) contain His/MBP-NAA10-WT or His/MBP-NAA10-V111G. The peaks corresponding to monomeric NAA10 (approximately at 75 mL) was further used for enzymatic experiments. b All fractions were analyzed by SDS-PAGE. c Time dependent Nt-acetylation of the substrate peptide EEEI24 catalyzed by His/MBP-NAA10-WT and His/MBP-NAA10-V111G. d Nt-acetylation of substrate peptides EEEI24 and DDDI24 catalyzed by His/MBP-NAA10-WT and His/MBP-NAA10-V111G. * statistically significant (p < 0.0001, student t-test)