Skip to main content

Advertisement

Table 2 Primer sequences for Sanger sequencing of novel mutations

From: Massive parallel sequencing as a new diagnostic approach for phenylketonuria and tetrahydrobiopterin-deficiency in Thailand

Primer name Sequence Tm (°C)
PAH_E6_F 5′-GAT GGC AGC TCA CAG GTT CT-3′ 60.5
PAH_E6_R 5′-CTT GTC TTC CCC TTC CCT CT-3′ 60.5
PTS_E5-6_F 5′-TGA TAA GGT GAG GTT TAG AGG C-3′ 60.1
PTS_E5-6_R 5′-CTC CAG AGC ACA ATG TGT ACG-3’ 61.2