Table 2 Primer sequences for Sanger sequencing of novel mutations
| Primer name | Sequence | Tm (°C) |
|---|---|---|
| PAH_E6_F | 5′-GAT GGC AGC TCA CAG GTT CT-3′ | 60.5 |
| PAH_E6_R | 5′-CTT GTC TTC CCC TTC CCT CT-3′ | 60.5 |
| PTS_E5-6_F | 5′-TGA TAA GGT GAG GTT TAG AGG C-3′ | 60.1 |
| PTS_E5-6_R | 5′-CTC CAG AGC ACA ATG TGT ACG-3’ | 61.2 |