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Table 1 List of primers used to amplify BRCA2 gene selected regions

From: Monoallelic characteristic-bearing heterozygous L1053X in BRCA2 gene among Sudanese women with breast cancer

PN* Primer’s nucleotide sequence PL* AT* Selected region Am*
A F: 5′ AGA CAC AGG TGA TAA ACA AG ‘3 20 50 3020 to 3380 361
R: 5′ CAA GGT ATT TAC AAT TTC AA ‘3 20 50   
B F:5′ GCT CTC TGA ACA TAA CAT TAA G ‘3 22 50 3281 to 3731 451
R: 5′ CAT TAT GAC ATG AAG ATC AG ‘3 20 50   
C F: 5′ TGA GAC CAT TGA GAT CAC AGC ‘3 21 55 4967 to 5673 707
R: 5′ TAG TCA CAA GTT CCT CAA CGC A ‘3 22 55   
  1. PN* Primer Name
  2. PL* Primer length in base pair
  3. AT* Annealing Temp
  4. Am* Amplicon size (bp)