Fig. 5

Gel shift assay of nuclear extract from endothelial cells showing a retarded band of protein-DNA, within the proximal promoter of Endoglin. Nuclear extract from endothelial cells (HMEC-1 cell line) was incubated with the double stranded biotinylated oligonucleotide, and in the presence of unspecific competitor (poli dI-dC), a retarded band could be detected (lane 2). However, the sample was specifically treated with 100× excess of the unlabeled double-stranded nucleotide (cold probe) (lane 3); when the mutated oligonucleotide was added, the binding efficiency of the probe was not diminished (lane 4). The experiment was repeated 4 times. This image is representative of the obtained results