Table 1 Primers used in RUNX2polymerase chain reaction (PCR)
From: A novel, complex RUNX2 gene mutation causes cleidocranial dysplasia
Positions | Forward nucleotide sequence (5′-3′) | Reverse nucleotide sequence (5′-3′) |
|---|---|---|
Exon 1 | AGAGAGAGAAAGAGCAAGGGG | GCATAGACTGTGGTTAGAGAGC |
Exon 2 | TTTCTTTGCTTTTCACATGTTACC | TGCTATTTGGAAAAGCTAGCAG |
Exon 3 | CGCTAACTTGTGGCTGTTGT | CGTGGGCAGGAAGACACC |
Exon 4 | CATTCCTGTCGGCCATTACTG | CATCAAAGGAGCCTAATGTGCT |
Exon 5 | AAGTGGTCATCGGAGGGTTT | TGCAGATAGCAAAGTCCACAA |
Exon 6 | GGCCACCAGATACCGCTTAT | CCAGCGTCTATGCAAGTGAA |
Exon 7 | GCCTGAAAGGATGGGGTTAT | CTGTGCAGGGATGGATTTTT |
Exon 8 | CTTATGGGCCTGCAGACTCT | AGTAACAACCAGACAGCCCA |
Exon 9 | CTGTGGCTTGCTGTTCCTTT | TGATACGTGTGGGATGTGGC |