Target | Direction | Primer sequence | Position on reference sequence | Product length (bp) | Annealing temp °C | Extension time (sec) |
---|---|---|---|---|---|---|
HBB Exon 1 and 2 | Forward | 5’-TGTCATCACTTAGACCTCACCCTG-3’ | 5,226,544 to 5,227,193 on NC_0000.11.10 | 686 | 58 | 40 |
Reverse | 5’-GGAAAGAAAACATCAAGCGTCCCATAG-3’ | |||||
HBB Intervening sequence 2 | Forward | 5’-TGCACGTGGATCCTGAGAACTTCA-3’ | 5,226,411 to 5,226,602 on NC_000011.10 | 228 | 56 | 20 |
Reverse | 5’-ACAGCAAATAAAAGAAACTAAAACGA-3’ | |||||
HBB Exon 3 | Forward | 5’-GCTGGATTATTCTGAGTCCAAGCTA-3’ | 5,225,508 to 5,225,797 on NC_000011.10 | 326 | 58 | 25 |
Reverse | 5’-TCAAGGCCCTTCATAATATCCCC-3’ | |||||
GAP-PCR Filipino β-thalassaemia deletion | Forward | 5’-GTAAATGAGTAAATGAAGGAATGAT-3’ | 5,112,653 to 5,232,062 on NC_000011.10 | 920 | 60 | 60 |
Reverse | 5’-TGTGATTTGGCTCTCTTCTTGTCTA-3’ |