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Fig. 4 | BMC Medical Genetics

Fig. 4

From: Rapid detection of pathological mutations and deletions of the haemoglobin beta gene (HBB) by High Resolution Melting (HRM) analysis and Gene Ratio Analysis Copy Enumeration PCR (GRACE-PCR)

Fig. 4

GRACE-PCR to determine copy numbers of the HBB gene. Limited cycle GRACE-PCR was used to amplify targets from the CLCN7 reference gene [34] and the HBB gene. The raw melt curves (a) are difficult to interpret, but after normalization (b) the ratio of CLCN7:HBB gene copy numbers is easily visualized

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BMC Medical Genetics

ISSN: 1471-2350

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