Skip to main content

Table 1 Specific primers sequences, restriction enzymes and restriction digestion products’ sizes for FVL, Prothrombin A20210G and MTHFR C677T. The PCR-RFLP products sizes are given according to the genotype polymorphisms

From: Associations of recurrent miscarriages with chromosomal abnormalities, thrombophilia allelic polymorphisms and/or consanguinity in Saudi Arabia

Gene

Length (bp)

Primer’s sequence

Restriction enzyme

Restriction digestion product size (bp)

References

Normal

Heterozygous

Homozygous

FVL

143

Forward: CATGAGAGACATCGCCTCTG

Reverse: GACCTAACATGTTCTAGCCAGAAG

MnII

25

37

81

25

37

81

118

25

118

[77–80]

Prothrombin

345

Forward: TCTAGAAACAGTTGCCTGGC

Reverse: ATAGCACTGGGAGCATTGAAGC

HindIII

345

23

322

345

23

322

MTHFR

198

Forward: TGAAGGAGAAGGTGTCTGCGGGA

Reverse: AGGACGGTGCGGTGAGAGTG

HinfI

198

23

175

198

23

175