Table 2 PCR primers and HRM conditions for scanning mutations of the entire ACTA2 coding region
From: Alpha-actin-2 mutations in Chinese patients with a non-syndromatic thoracic aortic aneurysm
Exon | Forward primer | Reverse Primer | Size (bp) | PCR Tm (°C) | HRM Tm (°C) |
|---|---|---|---|---|---|
Exon 2 | 5'-tgcccaattacagctgaggct-3' | 5'-tctgtgtcctgttatgttccaatca-3' | 233 | 58 | 82-87 |
Exon 3 | 5'-tttgggagatgctgactcataatgtg-3' | 5'-tgcatcctgagggcccaagct-3' | 208 | 58 | 80-85 |
Exon 4 | 5'-catcattgtgtttctcctctgtcc-3' | 5'-gaagtttccccagaccccacag-3' | 197 | 58 | 82-87 |
Exon 5 | 5'-tttgtcagatgggcaccttca-3' | 5'-gcgctccaaccagcttgctgtc-3' | 173 | 58 | 82-87 |
Exon 6 | 5'-ccagctgccatggtgacttatc-3' | 5'-cccctctcccccttatctccca-3' | 235 | 58 | 82-87 |
Exon 7-1 | 5'-cacctgtgcagaccctaatgtttg-3' | 5'-ggtctctgggcagcggaaac-3' | 220 | 58 | 80-85 |
Exon 7-2 | 5'-ccactgccgcatcctcatcct-3' | 5'-agacaatgactccccttcccag-3' | 164 | 58 | 82-87 |
Exon 8-1 | 5'-acttaaggaccatggcctgtgtc-3' | 5'-atcttcatggtgctgggtgctag-3' | 211 | 58 | 80-85 |
Exon 8-2 | 5'-aatgtcctatcagggggcacca-3' | 5'-gctgacactgctggcggc-3' | 144 | 58 | 82-87 |
Exon 9-1 | 5'-ctgcactaatgatgacattaatgacc-3' | 5'-ggagcaggaaagtgttttagaag-3' | 210 | 58 | 82-87 |
Exon 9-2 | 5'-cgggccttccattgtccac-3' | 5'-aatggtatcagtcgagtattgatcg-3' | 225 | 58 | 80-85 |