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Fig. 5 | BMC Medical Genetics

Fig. 5

From: Polymorphisms at the F12 and KLKB1 loci have significant trait association with activation of the renin-angiotensin system

Fig. 5

Co-localization of renin and KAL in a As4.1 (CRL-2193) juxtaglomerular cells and in b mouse kidney: The cells (upper two panels) and kidney sections (lower two panels) were stained with rabbit anti-KAL primary and donkey anti-rabbit IgG- Alexa Fluor®488 conjugated secondary (green); and goat anti-renin primary and donkey anti-goat IgG- Fluor®594 conjugated secondary (red). The nuclei were visualized with Hoechst 33342 dye (blue). A series of xy optical sections along the z-axis was acquired using a 60x oil immersion objective for cells and 20x objective for kidney section by deconvolution microscopy and the data set was processed to generate three-dimensional (3D) or representative xy section. Co-localization of KAL (green) and renin (red) is shown by overlay of the images (yellow fluorescence). Bar scale is 5 μM

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