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Fig. 5 | BMC Medical Genetics

Fig. 5

From: Tracking of the origin of recurrent mutations of the BRCA1 and BRCA2 genes in the North-East of Italy and improved mutation analysis strategy

Fig. 5

SNaPshot® analyses. a: Example of the multiplex and single amplified products; lane 1, 50 bp marker; lane 2 and 3, multiplex PCR of two different samples; lane 4, negative control of multiplex PCR; lane 5–12: single PCR products, in the order BRCA1 exon 3, 5, 11–1, 11–3, 20 and BRCA2 exon 17, 11-M, 22. b and c: Representative electropherograms of wild-type and mutated SNaPshot® reactions of BRCA1 (B) and BRCA2 (C). The nucleotides added by primer extension are shown in blue (G), black (C), red (T) and green (A);*, extension performed with an antisense primer

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