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Fig. 2 | BMC Medical Genetics

Fig. 2

From: Development and validation of a high throughput, closed tube method for the determination of haemoglobin alpha gene (HBA1 and HBA2) numbers by gene ratio assay copy enumeration-PCR (GRACE-PCR)

Fig. 2

GRACE-PCR assay, limited cycle multiplex PCR targeting both α-globin genes (HBA1 and HBA2) and a reference gene (CNCN7). Peak height, relative to the reference gene, in the melting plots (−dF/dT versus temperature) is proportional to the copy number of each gene (indicated in parenthesis under the gene name). Four genotypes with varying α-globin gene copy numbers are shown in plots a to d

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