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Table 2 Summary of WT and R205Q mutant thin filament functional data with and without human β-cardiac myosin S1

From: Establishing disease causality for a novel gene variant in familial dilated cardiomyopathy using a functional in-vitro assay of regulated thin filaments and human cardiac myosin

 

WT

R205Q

Effective Kd (μM)

1.2 ± 0.4

4.4 ± 0.5*

Effective k off (s−1) at 23 °C

118 ± 1

177 ± 3*

S1 ATPase pCa50

6.17 ± 0.01

5.70 ± 0.02*

S1 ATPase ∆pCa50

------

−0.47

% S1 Maximum Activity

100 ± 1 %

80 ± 3 %*

% S1 Minimum Activity

10 ± 1 %

8 ± 1 %

nH (S1 ATPase pCa Curve)

1.2 ± 0.1

1.1 ± 0.1

S1 ADP Release at 23 °C (s−1)

68 ± 1

67 ± 1

Tropomyosin binding, Kd (nM)

68 ± 6

48 ± 6

  1. Means ± SEM
  2. n represents: (1) for ANS, number of individual curves; ≥ 11 for Kd; ≥ 9 for k off : (2) for ATPase, number of individual pCa Curves; 33, WT; 11, R205Q; (3) for ADP release, number of individual curves; 8, WT; 6, R205Q; (4) for tropomyosin binding, number of individual curves, ≥ 3
  3. *p < 0.001 by Student t-test or if unequal variance, the Mann-Whitney rank sum test