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Fig. 3 | BMC Medical Genetics

Fig. 3

From: Establishing disease causality for a novel gene variant in familial dilated cardiomyopathy using a functional in-vitro assay of regulated thin filaments and human cardiac myosin

Fig. 3

Tropomyosin binding and steady state WT and R205Q thin filament pCa curves. a Binding of WT (closed circles) and R205Q (open circles) troponin complex to pyrene-labeled tropomyosin. b Calcium sensitivity of the ANS-labeled thin filament in the absence of S1 at 23 °C. c Calcium sensitivity of thin-filament-activated ATPase activity of human β-cardiac myosin S1 at 30 °C. For both (b) and (c), each curve is the average of >10 individual curves, and error bars represent SEM. Each curve was individually fit by the Hill equation, as summarized in Table 2. The shaded gray region represents the average physiological Ca2+ range during a heartbeat [23, 24]

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BMC Medical Genetics

ISSN: 1471-2350

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