Table 3 PCR conditions for genotyping: primer sequences, annealing temperatures and restrictions enzymes
From: Genetic polymorphisms associated with the inflammatory response in bacterial meningitis
SNP | Primer sequence | Annealing temperature | Restriction enzyme |
|---|---|---|---|
TNF -308G > A | F 5’AGGCAATAGGTTTTGAGGGCCAT'3 | 55 °C | NcoI |
R 5’TCCTCCCTGCTCCGATTCCG'3 | |||
TNF -857C > T | F 5’GGCTCTGAGGAATGGGTTAC'3 | 58 °C | TaiI |
R 5’CCTCTACATGGCCCTGTCTAC'3 | |||
IL8 -251A > T | F 5’CCATCATGATAGCATCTGTA'3 | 53 °C | AseI |
R 5’CCACAATTTGGTGAATTATTAA'3 |